Modified PCR methods for 3′ end amplification from serial analysis of gene expression (SAGE) tags
نویسندگان
چکیده
منابع مشابه
5′SAGE: 5′-end Serial Analysis of Gene Expression database
To comprehensively identify transcription start sites and the frequencies of individual mRNAs in human cell libraries, a method of 5' end Serial Analysis of Gene Expression (SAGE) was developed recently, which makes it possible to collect a large amount of start site information, and subsequently, we have established a related database server called 5'SAGE. This database displays the observed f...
متن کاملSerial Analysis of Gene Expression (SAGE) - Sequencing Errors
Serial Analysis of Gene Expression (SAGE) is a technique to study overall gene expression in different (normal or disease) tissues. Results take a form of a so-called SAGE library for each of the tissues studied. A SAGE library is a set of text-strings (typically 10base-pairs long), called tags. A tag is representative for a gene that is active in a particular cell or tissue. From a statistical...
متن کاملGeneration of longer cDNA fragments from serial analysis of gene expression tags for gene identification.
We have developed a technique called the generation of longer cDNA fragments from serial analysis of gene expression (SAGE) tags for gene identification (GLGI), to convert SAGE tags of 10 bases into their corresponding 3' cDNA fragments covering hundred bases. A primer containing the 10-base SAGE tag is used as the sense primer, and a single base anchored oligo(dT) primer is used as an antisens...
متن کاملAn evaluation of serial analysis of gene expression (SAGE) in the parasitic nematode, Haemonchus contortus.
This study evaluated a relatively new molecular technique, serial analysis of gene expression (SAGE), as a tool for quantifying gene expression in the ovine abomasal nematode Haemonchus contortus for which there is relatively limited (approximately 20% gene coverage) sequence information. SAGE technology generates data that are both qualitative and quantitative and, as such, compliments other f...
متن کاملUse of serial analysis of gene expression (SAGE) technology reveals new granulocytic markers.
Because of its short life span in blood, its low RNA content and its highly condensed nucleus, the granulocyte was initially considered as a terminally differentiated cell unable to express novel genes. However, mature granulocytes still contain a variety of mRNAs and may respond to external stimuli by rapid and complex changes in gene expression. The present work was undertaken to provide a wi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Journal
سال: 2009
ISSN: 1742-464X,1742-4658
DOI: 10.1111/j.1742-4658.2009.06981.x